解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The major mono- and disialogangliosides of the extensively characterized established human glioma line D54MG were isolated and purified from subcutaneous solid xenografts grown in athymic (nu/nu) mice. Structural determination showed that they belonged to the lactotetraosylceramide series. The sialyllactotetraosylcera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80580-4
更新日期:1986-05-26 00:00:00
abstract::The possible relationship between pyridoxal phosphate-dependent ornithine decarboxylase (ODC) activity and glucose-6-phosphate dehydrogenase (G6PD) activity has been studied in the osteoblasts of the growth-plate of metatarsals of rats fed a pyridoxine-deficient diet, which caused depressed G6PD levels. The G6PD activ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80579-8
更新日期:1986-05-26 00:00:00
abstract::The Ca2+-binding parvalbumin has been purified for the first time from rat kidney. Its biochemical and immunological properties were indistinguishable from the muscle counterpart. By immunohistochemical methods parvalbumin was localized in part of the distal tubule and proximal collecting duct, similar to the vitamin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80569-5
更新日期:1986-05-26 00:00:00
abstract::Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+,Cl- transporter. We found that Hb SS, SC a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80538-5
更新日期:1986-05-05 00:00:00
abstract::Microdissected, beta-cell-rich pancreatic islets from ob/ob mice were used in studies of 86Rb+ transport. D-Glucose (20 mM) induced a biphasic reduction in 86Rb+ efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86Rb+ uptake (5 min) was dose-dependently reduced by ou...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80512-9
更新日期:1986-05-05 00:00:00
abstract::Measurements of the effects of NH3/NH4+ on glucose-induced electrical activity in beta-cells from microdissected mouse islets of Langerhans and on intracellular pH in single collagenase-isolated islets pre-loaded with a fluorescent pH probe were performed and are reported here. Application of NH3/NH4+ (15 mM) in the p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80539-7
更新日期:1986-05-05 00:00:00
abstract::Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80472-0
更新日期:1986-04-21 00:00:00
abstract::After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80491-4
更新日期:1986-04-21 00:00:00
abstract::The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had bee...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80408-2
更新日期:1986-03-31 00:00:00
abstract::Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80427-6
更新日期:1986-03-31 00:00:00
abstract::Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80412-4
更新日期:1986-03-31 00:00:00
abstract::Cloning and nucleotide sequence analysis of the human calcitonin mRNA from the BEN lung carcinoma cell line, a cell line known to secrete high-Mr forms of calcitonin, showed no difference in the coding region at the nucleotide level compared with calcitonin mRNA isolated from medullary thyroid carcinoma which secretes...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81187-5
更新日期:1986-03-17 00:00:00
abstract::Creutzfeldt-Jakob disease is a slow, infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide- or 5-hydroxytryptamine-mediated activation of adenylate cyclase. Thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81205-4
更新日期:1986-03-17 00:00:00
abstract::The sulfoxide Ro 18-5364, a potential metabolite of the IND Ro 18-5362, is a powerful inhibitor of gastric mucosal (H+ + K+)-ATPase, decreasing enzymatic activity with an apparent Ki of 0.1 microM. Exposure of Ro 18-5364-treated gastric membranes to dithiothreitol fully restored (H+ + K+)-ATPase activity. ATP protecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80324-6
更新日期:1986-03-03 00:00:00
abstract::The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80350-7
更新日期:1986-03-03 00:00:00
abstract::Post-absorptive gluconeogenesis from lactate measured in vivo increases 3-fold in 24-month-old rats compared to 3-month-old animals. Fractional lactate turnover rates showed no significant differences between the two groups of animals. Lower plasma glucose concentrations and insulin-glucagon ratios may explain the inc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80318-0
更新日期:1986-03-03 00:00:00
abstract::The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80306-4
更新日期:1986-03-03 00:00:00
abstract::The effect of platelet-activating factor (PAF) on polyphosphoinositide metabolism and 45Ca2+ efflux was examined in a vascular smooth muscle cell line (A7r5). PAF stimulated a rapid but transient production of inositol trisphosphate and inositol bisphosphate which, in the presence of lithium, resulted in an accumulati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80288-5
更新日期:1986-03-03 00:00:00
abstract::The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80300-3
更新日期:1986-03-03 00:00:00
abstract::Two polypeptides antigenically related to mammalian HMG1/2 have been detected in the yeast Saccharomyces cerevisiae. One exhibits an electrophoretic mobility in the range of mammalian HMG1/2 whereas the second polypeptide comigrates with yeast HMG S4. Evidence for the presence of HMG14/17 in yeast has not been obtaine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80322-2
更新日期:1986-03-03 00:00:00
abstract::50 S ribosomal subunit derivatives without the 5 S RNA-protein complex obtained either by splitting with EDTA or by reconstitution from the 23 S RNA and proteins have been studied by electron microscopy. Removal of the 5 S RNA-protein complex is shown to affect neither the overall morphology of the larger ribosomal su...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80302-7
更新日期:1986-03-03 00:00:00
abstract::The vasopressin precursor is composed of 3 domains in line, namely vasopressin, MSEL-neurophysin and a glycopeptide referred to as copeptin, which are separated during the processing. In guinea pig neurohypophysis, the precursor is partially processed so that a two-domain fragment, MSEL-neurophysin--copeptin, can be f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80320-9
更新日期:1986-03-03 00:00:00
abstract::A trisialoganglioside not found before in human tissue was isolated from showed this to be II3(NeuAc)3-LacCer or GT3. The ganglioside constituted 40% of the trisialogangliosides of human lung or 0.2 nmol ganglioside per g tissue. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80259-9
更新日期:1986-02-17 00:00:00
abstract::Rabbits ingesting 4-fluorophenylalanine are known to incorporate small amounts of this fluorinated amino acid into their proteins. Carbonic anhydrase I isolated from the erythrocytes of animals so maintained exhibits a well-resolved fluorine NMR signal for each phenylalanine in the sequence. The chemical shifts of mos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80216-2
更新日期:1986-02-03 00:00:00
abstract::The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80204-6
更新日期:1986-02-03 00:00:00
abstract::The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80227-7
更新日期:1986-02-03 00:00:00
abstract::o-Aminophenol was found to be rapidly metabolized to a brown compound in the presence of purified human oxy- and methemoglobin, coupled with the oxidation and reduction of these hemoglobins by o-aminophenol. The final product of o-aminophenol was identified as 2-aminophenoxazine-3-one, by using spectrophotometry and H...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80211-3
更新日期:1986-02-03 00:00:00
abstract::In guinea pig caudate slices preloaded with [3H]choline, superfusion with medium containing Ca2+ and the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate) produced an increase in the Ca2+-dependent, depolarization-evoked release of acetylcholine (ACh), with no significant effect on the evoked Ca2+ influx. The ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80146-6
更新日期:1986-01-20 00:00:00
abstract::Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80120-x
更新日期:1986-01-20 00:00:00
abstract::1,2-Didecanoylglycerol (diC10) is taken up by human platelets and sequentially converted to 1,2-didecanoylphosphatidic acid (PA10) and 1,2-didecanoylphosphatidylinositol (PI10). Agents that increase cyclic AMP in platelets, such as prostacyclin and forskolin, sequentially convert diC10 to PA10 and PI10. They decrease ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80141-7
更新日期:1986-01-20 00:00:00
abstract::A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80170-3
更新日期:1986-01-20 00:00:00
abstract::The activity of calcium, phospholipid-dependent protein kinase (PKc), which is thought to play an important role in cell proliferation, has been measured in the particulate and soluble fractions of cultured cells, under different proliferative conditions. Our results indicate that proliferating cells display higher PK...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80192-2
更新日期:1986-01-20 00:00:00
abstract::The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80102-8
更新日期:1986-01-06 00:00:00
abstract::We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80106-5
更新日期:1986-01-06 00:00:00
abstract::It is shown that conformational changes of receptor proteins brought about by binding of a ligand induce changes in the lipid environment of the receptor that can be monitored by fluorescent lipid probes. On this basis a new approach to studies of ligand-receptor binding is proposed. Using the interaction of the ricin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80108-9
更新日期:1986-01-06 00:00:00
abstract::During lactation glucose metabolism in paraovarian adipocytes is characterized by a 40 and 80% decrease of glucose incorporation into CO2 and fatty acids in the presence of insulin. In contrast with the stimulation by insulin of glucose incorporation into lactate, glycerol remains unchanged. As a result, insulin sensi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80103-x
更新日期:1986-01-06 00:00:00
abstract::The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80040-0
更新日期:1986-01-01 00:00:00
abstract::The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80046-1
更新日期:1986-01-01 00:00:00
abstract::Affinity labelling of pancreatic RNase with 4-(N-2-chloroethyl-N-methylamino)benzylamide and (N----P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as sugg...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80052-7
更新日期:1986-01-01 00:00:00
abstract::The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80161-7
更新日期:1985-12-02 00:00:00